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KMID : 0525720110160030204
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Journal of Chitin and Chitosan
2011 Volume.16 No. 3 p.204 ~ p.208
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The Effect of Low Molecular Weight Chitosan Oligosaccharide on MMP-9 Activity and TIMPs Expression in Human Breast Cancer Cell
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Shin Jin-Sun
Lee Kyu-Shik
Nam Kyung-Soo
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Abstract
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We investigated the effect of low molecular weight chitosan oligosaccharide (COS-L, MW<1,000 Da) on metastatic activity in MDA-MB-231 human breast cancer cells. In a concentration range of 0.05 to 3 mg/mL, cell migration was significantly decreased by COS-L in a dose dependent manner. Matrix metalloproteinase-9 (MMP-9) activity induced by phorbol-12-myristate-13-acetate (TPA) was critically inhibited by COS-L at 3 mg/mL. In contrast, tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) transcription was dose-dependently increased. However, tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) was not changed. The results suggest that the decrease of metastatic potential in MDA-MB-231 human breast cancer call by COS-L should be mediated by the inhibition of MMP-9 activity via the induction of TIMP-1. In conclusion, present investigation provides critical information that COS-L may diminish the risk of mortality and relapse caused by metastasis in breast cancer.
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KEYWORD
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Chitosan oligosaccharide, Breast cancer, Matrix metalloproteinase-9, Tissue inhibitor of metalloproteinases, Metastasis
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